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K. Maeki and S.A. Ae (1966)
A chromosome study of twenty-eight species of Himalayan butterflies (Papilionidae, Pieridae).
Spec. Bull. Lep. Soc. Jap., N0.2:107-119 (1966).

Summary

1. The chromosome numbers and their sizes are reported of 12 species of the Papillionidae and 16 species of the Pieridae from Nepal. Of these, 14 species were cytologically studied for the first time. All the chromosome counts wre carried out on the basis of the spermatocyte.

2. 3.

Karyotype of Papilio polytes romulus Cramer (n, 30, I). I : primary spermatocyte (n=30).

The haploid chromosome-number is 30. Counts were made in 3 nuclei (I) in the testes of a male taken by A. Hara between Dumuhan and Goldiagong on August 3 (H-65). All the chromosomes are similar in size. The authors also examined the testes from the other 3 specimens, but no dividing cells were obserbed (H-74, H-79, H-80). Maeki, Ogata, and Shirozu ('65) reported the same features of the karyotype in P. polytes pasikrates from Formosa.

As a fixative, Allen's PFA-3 solution only was used. The sections, 10 micra in thickness, were made according to the ordinary paraffin method and stained with Heidemnhain's iron-hematoxyline with counterstaining of light green. Camera lucida drawings were made at the magnification of 4200 diameters. The photomicrographs were taken by means of a MIKAS camera.