Medakafish developmental stage map

　　　　　

	1	2	3	4	5
0	Unfertilized eggs
1	Activated egg	Blastodisc	2 cell	4 cell	8 cell
2	16 cell	32 cell	Early morula	Late morula	Early blastula
3	Late blastula	Pre-early gastrula	Early gastrula	Pre-mid gastrula	Mid gastrula
4	Late gastrula	Early neurula	Late neurula	2 somite	4 somite
5	6 somite	9 somite	12 somite	16 somite	18-19 somite
6	22 somite	24 somite	30 somite	34 somite	35 somite
7	Gill vessel formation	Somite completion	Notochord vacuolization	Pectoral fin circulation	Visceral vessels
8	Heart development	Pericardial cavity	Spleen development	Hatching	1st fry
9	2nd fry	3rd fry	1st young fish	2nd young fish

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Stage 0 Unfertilized eggs

Stage 1 (3 min) Activated egg stage

Stage 2 Blastodisc stage

Stage 3 (1 hr 5 min) 2 cell stage

Stage 4 (1 hr 45 min) 4 cell stage

Stage 5 (2 hrs 20 min) 8 cell stage

Stage 6 (2 hrs 55 min) 16 cell stage

Stage 7 (3 hrs 30 min) 32 cell stage

Stage 8 (4 hrs 5 min) Early morula stage

Stage 9 (5 hrs 15 min) Late morula stage

Stage 10 (6 hrs 30 min) Early blastula stage

Stage 11 (8 hrs 15 min) Late blastula stage

Stage 12 (10 hrs 20 min) Pre-early gastrula stage

Stage 13 (13 hrs) Early gastrula stage

Stage 14 (15 hrs) Pre-mid gastrula stage

Stage 15 (17 hrs 30 min) Mid gastrula stage

Stage 16 (21 hrs ) Late gastrula stage

Stage 17 (1 day 1 hr) Early neurula stage (Head formation)

Stage 18 (1 day 2 hrs) Late neurula stage (Optic bud formation)

Stage 19 (1 day 3 hrs 30 min) 2 somite stage

Stage 20 (1 day 7 hrs 30 min) 4 somite stage

Stage 21 (1 day 10 hrs) 6 somite stage (Brain and otic vesicle formation)

Stage 22 (1 day 14 hrs) 9 somite stage (Appearance of heart anlage)

Stage 23 (1 day 17 hrs) 12 somite stage (Formation of tubular heart)

Stage 24 (1 day 20 hrs) 16 somite stage (Start of heart beating)

Stage 25 (2 days 2 hrs) 18-19 somite stage (Onset of blood circulation)

Stage 26 (2 days 6 hrs) 22 somite stage (Guanophore development & notochord vacuolization)

Stage 27 (2 days 10 hrs) 24 somite stage (Appearance of pectoral fin bud)

Stage 28 (2 days 16 hrs) 30 somite stage (Onset of retinal pigmentation)

Stage 29 (3 days 2 hrs) 34 somite stage (Internal ear formation)

Stage 30 (3 days 10 hrs) 35 somite stage (Blood vessel development)

Stage 31 (3 days 23 hrs) Gill blood vessel formation stage

Stage 32 (4 days 5 hrs) Somite completion stage (Formation of pronephros and air bladder)

Stage 33 (4 days 10 hrs) Stage at which notochord vacuolization is completed

Stage 34 (5 days 1 hr) Pectoral fin blood circulation stage

Stage 35 (5 days 12 hrs) Stage at which visceral blood vessels form

Stage 36 (6 days) Heart development stage

Stage 37 (7 days) Pericardial cavity formation stage

Stage 38 (8 days) Spleen development stage (Differentiation of caudal fin begins)

Stage 39 (9 days) Hatching stage

Stage 40 1st fry stage

Stage 41 2nd fry stage

Stage 42 3rd fry stage

Stage 43 1st young fish stage

Stage 44 2nd young fish stage

By the Nagoya University Medakafish Group

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Other information of interest may be found in;

Iwamatsu, T. (1994): Stages of normal development in the medaka Oryzias latipes.; Zool. Sci. , 11 : 825-839 (1994).

Yamamoto T (1975): Stages in the development.; In "Medaka (Killifish): Biology and Strains"(Yamamoto T ed), pp. 59-72.
Keigaku Publ Co, Tokyo.